DNA
Part:BBa_K4693003:Design
Designed by: Vibhavari Medepalli Group: iGEM23_IISER-TVM (2023-10-10)
Truncated cortisol aptamer
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This aptamer was modified with a fluorescein based fluorophore FAM at the 5' end. It has an excitation at 490nm with an maximum emission at 520nm. This modified aptamer was obtained via IDT.
Source
Sharma, V., Sharma, T. K., & Kaur, I. (2023). Electrochemical detection of cortisol using a structure-switching aptamer immobilized on gold nanoparticles-modified screen-printed electrodes. Journal of Applied Electrochemistry, 1-12.